| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Evaluation of dot-blot test for serological diagnosis of bovine brucellosis
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| Autores: |
Bastos,Carla Resende
Mathias,Luis Antonio
Jusi,Márcia Mariza Gomes
Santos,Renata Ferreira dos
Silva,Glaucenyra Cecília Pinheiro da
André,Marcos Rogério
Machado,Rosangela Zacarias
Bürger,Karina Paes
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| Data: |
2018-09-01
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| Ano: |
2018
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| Palavras-chave: |
Brucella abortus
Animal health
Zoonoses
2-Mercaptoethanol
Complement fixation test
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| Resumo: |
Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the conjugate at 1:30,000. The comparison of the dot-blot results with 2-ME showed Kappa index of 0.9939, sensitivity of 99.48%, and specificity 99.91%, with CF, Kappa index of 0.8226, sensitivity 100% and specificity 95.32%. Using the combination of the test results 2-ME and CF to establish the true condition of the animal, the dot-blot showed relative sensitivity of 100%, and relative specificity of 99.91%. The evaluated test proved to be effective and reliable, besides being easy to handle and interpret the results.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300564
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1016/j.bjm.2017.10.002
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.49 n.3 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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